Role of spoligotyping and IS6110-RFLP in assessing genetic diversity of Mycobacterium tuberculosis in India

Mathuria, Jitendra Prasad ; Sharma, Pragya ; Prakash, Pradyot ; Samaria, Jai Kumar ; Katoch, Vishwa Mohan ; Anupurba, Shampa (2008) Role of spoligotyping and IS6110-RFLP in assessing genetic diversity of Mycobacterium tuberculosis in India Infection, Genetics and Evolution, 8 (3). pp. 346-351. ISSN 1567-1348

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S15671...

Related URL: http://dx.doi.org/10.1016/j.meegid.2008.02.005

Abstract

In the present study, genetic diversity analysis of Mycobacterium tuberculosis isolated from patients attending a tertiary care hospital, North India, has been attempted. Eighty three isolates of M. tuberculosis were subjected to DNA fingerprinting using spoligotyping and IS6110-RFLP techniques. Spoligotype patterns showed that central Asian (32.5%), ill defined T (13.2%) and Beijing (10.8%) families were predominant in ongoing transmission of the bacterium. Two STs; ST26 (CAS_Delhi) and ST1 (Beijing) represented 36.1% of the total M. tuberculosis population in eastern Uttar Pradesh, North India. IS6110 RFLP analysis showed that isolates having low and zero copy number of the IS element were 15.6% and 19.2%, respectively. Out of the 47 isolates clustered by spoligotyping, 40 could be further differentiated as unique strains by IS6110-RFLP. Therefore, this study recommends that both the techniques be used simultaneously for DNA fingerprinting of M. tuberculosis in India.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Tuberculosis; Spoligotyping; IS6110-RFLP; Genetic Diversity
ID Code:16670
Deposited On:15 Nov 2010 13:29
Last Modified:22 Feb 2011 06:16

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