Chugh, Jeetender ; Hosur, Ramakrishna V. (2008) Spectroscopic labeling of A, S/T in the 1H-15N HSQC spectrum of uniformly (15N-13C) labeled proteins Journal of Magnetic Resonance, 194 (2). pp. 289-294. ISSN 1090-7807
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S10907...
Related URL: http://dx.doi.org/10.1016/j.jmr.2008.07.022
Abstract
A new triple resonance two-dimensional experiment, termed (HC)NH, has been described to generate specific labels on the peaks of alanines and serines/threonines, separately, in the 1H-15N HSQC spectrum of a protein. The performance of the pulse sequence has been demonstrated with a 151 residue protein. The method permits the investigation of local environments around those specific residues without actually having to obtain complete resonance assignments for the entire protein. With this one can envisage use of the technique for studying large protein systems from different points of view.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Band-selective Pulse; Alanine Labels; Serine; Threonine Labels; NMR |
| ID Code: | 16610 |
| Deposited On: | 15 Nov 2010 13:34 |
| Last Modified: | 03 Jun 2011 08:37 |
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