Generation of serine/threonine check points in HN(C)N spectra

Kumar, Dinesh ; Chugh, Jeetender ; Hosur, Ramakrishna V. (2009) Generation of serine/threonine check points in HN(C)N spectra Journal of Chemical Sciences, 121 (6). pp. 955-964. ISSN 0253-4134

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Official URL: http://www.ias.ac.in/chemsci/Pdf-Nov2009/955.pdf

Related URL: http://dx.doi.org/10.1007/s12039-009-0124-6

Abstract

We describe here a simple modification of the HN(C)N experiment for the generation of serine/threonine check points in the three-dimensional experiment. The various 'triplet of residue' specific peak patterns in the spectra are documented for ease of analysis and sequential backbone resonance assignment. The performance of this experiment, referred to as HN(C)N-ST, is demonstrated using two proteins, one properly folded and the other completely denatured. It is noteworthy that, even in the denatured protein, where spectral dispersions are rather poor, about 90% of the sequential connectivities through the chain could be established from this single experiment. This would have great implications for structural genomics efforts.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:NMR- N and C Labelled Protein; Resonance Assignment; Band-selective Pulse; Check Points; HN(C)N; HN(C)N-ST
ID Code:16585
Deposited On:15 Nov 2010 09:31
Last Modified:17 May 2016 01:19

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