Agarwal, Sangeeta ; Grover, Anil (2005) Isolation and transcription profiling of low-O2 stress-associated cDNA clones from the flooding-stress-tolerant FR13A rice genotype Annals of Botany, 96 (5). pp. 831-844. ISSN 0305-7364
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Official URL: http://aob.oxfordjournals.org/content/96/5/831.abs...
Related URL: http://dx.doi.org/10.1093/aob/mci233
Abstract
Background and Aims: Flooding stress leads to a significant reduction in transcription and translation of genes involved in basal metabolism of plants. However, specific genes are noted to be up-regulated in this response. With the aim of isolating genes that might be specifically involved in flooding stress-tolerance mechanism(s), two subtractive cDNA libraries for the flooding-stress-tolerant rice genotype FR13A have been constructed, namely the single and double subtraction libraries (SSL and DSL, respectively). Methods: To construct the SSL, mRNAs present in the unstressed control FR13A roots were subtracted from the mRNA pool present in low O2-stressed roots of FR13A rice seedlings. The DSL was constructed from mRNAs isolated from the roots of low O2-stressed FR13A rice seedlings from which pools of low-O2-stress up-regulated mRNAs from Pusa Basmati 1 and constitutively expressed mRNAs from FR13A roots were subtracted. Results: In all, 400 and 606 cDNA clones were obtained from the SSL and DSL, respectively. Global transcript profiling by reverse northern analysis revealed that a large number of clones from these libraries were up-regulated by anaerobic stress. Importantly, selective up-regulated clones showed characteristic cultivar- and tissue-specific expression profiles. Sequencing and annotation of the up-regulated clones revealed that specific signal proteins, hexose transporters, ion channel transporters, RNA-binding proteins and transcription factor proteins possibly play important roles in the response of rice to flooding stress. Also a significant number of novel cDNA clones was noted in these libraries. Conclusions: It appears that cellular functions such as signalling, sugar and ion transport and transcript stability play an important role in conferring higher flooding tolerance in the FR13A rice type.
Item Type: | Article |
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Source: | Copyright of this article belongs to Oxford University Press. |
Keywords: | Low O2; Flooding Stress; FR13A; Pusa Basmati 1; Rice Subtraction Library |
ID Code: | 15786 |
Deposited On: | 13 Nov 2010 12:33 |
Last Modified: | 17 May 2016 00:39 |
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