Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera

Pareek, Ashwani ; Singla, Sneh Lata ; Grover, Anil (1995) Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera Plant Molecular Biology, 29 (2). pp. 293-301. ISSN 0167-4412

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Official URL: http://www.springerlink.com/content/m34r6445056585...

Related URL: http://dx.doi.org/10.1007/BF00043653

Abstract

Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in rice seedlings subjected to salinity (NaCl), water stress, low-temperature stress and exogenous abscisic acid application. These proteins also accumulated when rice seedlings grown in pots under natural conditions were subjected to water stress by withholding watering. Seedlings of Triticum aestivum, Sorghum bicolor, Pisum sativum, Zea mays, Brassica juncea and mycelium of Neurospora crassa showed accumulation of the immunological homologues of both the 104 and the 90 kDa polypeptides, in response to high-temperature stress. We have earlier shown that shoots of rice seedlings exposed to heat shock accumulate a 110 kDa polypeptide which is an immunological homologue of the yeast HSP 104 (Singla and Grover, Plant Mol Biol 22: 1177-1180, 1993). Employing anti-rice HSP 104 antibodies and anti-yeast HSP 104 antibodies together, we provide evidence that rice HSP 104 is different from the earlier characterized rice HSP 110.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Heat Shock Proteins; Immunological Homologues; Oryza sativa; Plant Genera; Stress Proteins
ID Code:15682
Deposited On:13 Nov 2010 12:44
Last Modified:15 Feb 2011 07:47

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