Differentiation responses in callus cultures of Datura innoxia by phospholipid precursors

Abstract

The lipid composition of callus cultures of Datura innoxia grown on media supplemented with phospholipid precursors was studied. Choline or ethanolamine supplementation inhibited greening and shoot differentiation and led to an increase in the relative levels of the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and to a decrease in the levels of galactolipids. The relative level of phosphatidylinositol which exhibited an inverse relationship with the degree of differentiation, was increased to a level comparable to that of the non-green undifferentiated callus.