Phytochrome in control of differentiation and phosphatidylinositol turnover in Brassica oleracea cultures

Abstract

Red-light-induced proliferation and far-red induced differentiation and red/far-red reversibility indicated the involvement of phytochrome in control of differentiation in Brassica oleracea cultures. An increase in the level of glycerophosphatidylinositol with a concomitant decrease in glycerophosphatidylinositol phosphate and glycerophos-phatidylinositol biphosphate were obtained in the light grown seedlings as compared to the dark grown ones. A saturating pulse of red light, stimulated glycerophosphatidylinositol. Subsequent irradiation of red-light-treated seedlings with far-red light, decreased glycerophosphatidylinositol and increased glycerophosphatidylinositol phosphate and glycerophosphatidylinositol biphosphate and the photoreversibility was lost by an insertion of a dark incubation between red and far-red treatments.