Shary, Semarjit ; Guha-Mukherjee, Sipra (2004) Isolation and expression studies of differentiation-specific genes in tobacco dihaploids using PCR-based subtractive hybridization method Plant Science, 166 (2). pp. 317-322. ISSN 0168-9452
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01689...
Related URL: http://dx.doi.org/10.1016/j.plantsci.2003.09.021
Abstract
The method of subtractive suppression hybridization has been used to detect and clone differentially expressed genes during differentiation. For this, we used dihaploid tobacco plants as starting material and compared mRNA from proliferating callus with that of differentiating callus. The dihaploid homozygous plants were chosen to get the expression of rare genes which are otherwise difficult to detect in diploid plants. The cDNAs after subtraction were cloned and a total of 41 unique clones were identified which are upregulated in differentiating tissue, among these 21 were novel while 20 showed homology with different genes in database. An explanation has been given regarding the role of these genes in the developmental process.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Differentiation; Subtractive Hybridization; Differential Screening; Organogenesis; Tobacco (Nicotiana tabacum) |
| ID Code: | 15583 |
| Deposited On: | 13 Nov 2010 12:53 |
| Last Modified: | 17 Feb 2011 04:44 |
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