Synthesis and LB film formation of fatty acid- fad complex

Abstract

Flavin adenine dinucleotide can exist in two oxidation states. Switching from one state to the other can be achieved chemically or electrically. The flavin undergoes reduction reversibly by two one electron steps or one two electron step, changing the chemistry of the enediamine subfunction of the isoalloxazine moiety. The adenine moiety is not involved in the catalysis, but helps in anchoring the coenzyme at the active site. The hydrophilicity of FAD has been changed by chemically attaching hydrocarbon chains to the noncatalytic adenine moiety. The modified coenzyme has been verified to retain the electro chemical, optical and the biochemical properties. The amphiphilic molecules can form monolayers at the air-water interface. A film of desired thickness can be formed on a solid support by means of monomolecular deposition using LB film technique. The characterization of the film has been done using spectrophotometric and electrochemical methods.