Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells

Madesh, Muniswamy ; Ramachandran, Anup ; Balasubramanian, K. A. (1999) Nitric oxide prevents anoxia-induced apoptosis in colonic HT29 cells Archives of Biochemistry and Biophysics, 366 (2). pp. 240-248. ISSN 0003-9861

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Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in carcinogenesis. The aim of the present study was to investigate anoxia-induced cell death in colon-derived HT29 cells and the effect of nitric oxide on this phenomenon. It was found that HT29 cells subjected to anoxia undergo apoptosis in a time dependent manner, as determined by DNA fragmentation and Hoechst-33258 dye staining. Cytochrome c release from mitochondria to cytosol is a key step in this process and this release precedes DNA fragmentation. NO inhibits anoxia induced apoptosis in these cells by inhibiting the release of cytochrome c and thus may play a role in modulating the apoptotic cell death of colon-derived epithelial cells.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Nitric Oxide; Apoptosis; Cytochrome c; HT29 Cells
ID Code:1462
Deposited On:05 Oct 2010 12:28
Last Modified:14 May 2011 04:54

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