Crystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase fromXanthomonas oryzaepv.oryzae

Kumar, Sushil ; Haque, Asfarul S. ; Jha, Gopaljee ; Sonti, Ramesh V. ; Sankaranarayanan, Rajan (2012) Crystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase fromXanthomonas oryzaepv.oryzae Acta Crystallographica Section F Structural Biology and Crystallization Communications, 68 (10). pp. 1191-1194. ISSN 1744-3091

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Official URL: https://doi.org/10.1107/S1744309112034197

Related URL: http://dx.doi.org/10.1107/S1744309112034197

Abstract

The bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. The secreted exoglucanase CbsA is an important virulence factor of this pathogen. It belongs to the glycosyl hydrolase 6 family of proteins based on the carbohydrate-active enzyme (CAZY) classification. In this study, CbsA has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.86 Å and belonged to space group P212121. It contained one monomer per asymmetric unit, with a solvent content of 45.8%.

Item Type:Article
Source:Copyright of this article belongs to International Union of Crystallography.
Keywords:Type II secretion system; Exoglucanases; Oryza sativa; Bacterial leaf blight.
ID Code:141867
Deposited On:27 Dec 2025 11:47
Last Modified:27 Dec 2025 11:47

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