An endocytic pathway as a target of tubby for regulation of fat storage

Mukhopadhyay, Arnab ; Pan, Xiaojing ; Lambright, David G ; Tissenbaum, Heidi A (2007) An endocytic pathway as a target of tubby for regulation of fat storage EMBO reports, 8 (10). pp. 931-938. ISSN 1469-221X

Full text not available from this repository.

Official URL: http://doi.org/10.1038/sj.embor.7401055

Related URL: http://dx.doi.org/10.1038/sj.embor.7401055

Abstract

The tubby loci provide a unique opportunity to study adult-onset obesity. Mutation in either mammalian tubby or its homologue in Caenorhabditis elegans, tub-1, results in increased fat storage. Previously, we have shown that TUB-1 interacts with a new Rab GTPase-activating protein, RBG-3, for the regulation of fat storage. To understand further the molecular mechanism of TUB-1, we identified the Rab GTPase downstream of RBG-3. We found that RBG-3 preferentially stimulates the intrinsic GTPase activity of RAB-7 in both human and C. elegans. Importantly, either mutation or RNA interference knockdown in rab-7 reduces stored fat in wild type and tub-1 mutants. In addition, the small GTPase rab-5 and genes that regulate Rab membrane localization and nucleotide recycling are required for the regulation of fat storage, thereby defining a role for endocytic recycling in this process. We propose that TUB-1 controls receptor or sensory molecule degradation in neurons by regulating a RAB-7-mediated endocytic pathway.

Item Type:Article
Source:Copyright of this article belongs to European Molecular Biology Organization.
ID Code:136570
Deposited On:24 Jun 2025 09:58
Last Modified:24 Jun 2025 09:58

Repository Staff Only: item control page