Sarkar, Gautam Chandra ; Rautela, Umanshi ; Goyala, Anita ; Datta, Sudeshna ; Anand, Nikhita ; Singh, Anupama ; Singh, Prachi ; Chamoli, Manish ; Mukhopadhyay, Arnab (2023) DNA damage signals from somatic uterine tissue arrest oogenesis through activated DAF-16 Development, 150 (17). ISSN 0950-1991
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Official URL: http://doi.org/10.1242/dev.201472
Related URL: http://dx.doi.org/10.1242/dev.201472
Abstract
Germ line integrity is crucial for progeny fitness. Organisms deploy the DNA damage response (DDR) signaling to protect the germ line from genotoxic stress, facilitating the cell-cycle arrest of germ cells and DNA repair or their apoptosis. Cell-autonomous regulation of germ line quality in response to DNA damage is well studied; however, how quality is enforced cell non-autonomously on sensing somatic DNA damage is less known. Using Caenorhabditis elegans, we show that DDR disruption, only in the uterus, when insulin/IGF-1 signaling (IIS) is low, arrests oogenesis in the pachytene stage of meiosis I, in a FOXO/DAF-16 transcription factor-dependent manner. Without FOXO/DAF-16, germ cells of the IIS mutant escape the arrest to produce poor-quality oocytes, showing that the transcription factor imposes strict quality control during low IIS. Activated FOXO/DAF-16 senses DDR perturbations during low IIS to lower ERK/MPK-1 signaling below a threshold to promote germ line arrest. Altogether, we elucidate a new surveillance role for activated FOXO/DAF-16 that ensures optimal germ cell quality and progeny fitness in response to somatic DNA damage.
Item Type: | Article |
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Source: | Copyright of this article belongs to Company of Biologists Limited. |
Keywords: | FOXO/DAF-16; Insulin Signaling; Pachytene Arrest; Germ Line; DNA Damage Response |
ID Code: | 136533 |
Deposited On: | 24 Jun 2025 09:41 |
Last Modified: | 24 Jun 2025 09:41 |
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