Misra, Sanchaita ; Ikbal, Abu Md Ashif ; Bhattacharjee, Dipanjan ; Hore, Minakshi ; Mishra, Snehasis ; Karmakar, Sankha ; Ghosh, Alakendu ; Srinivas, Ravurushanmuk ; Das, Abhik ; Agarwal, Shivankar ; Saha, Krishna Das ; Bhardwaj, Prashant ; Ubhadia, Ishvarlal Bhudarbhai ; Ghosh, Parasar ; De, Sirshendu ; Tiwari, Onkar Nath ; Chattopadhyay, Debprasad ; Palit, Partha (2022) Validation of antioxidant, antiproliferative, and in vitro anti‐rheumatoid arthritis activities of epigallo‐catechin‐rich bioactive fraction from Camellia sinensis var. assamica , Assam variety white tea, and its comparative evaluation with green tea fraction Journal of Food Biochemistry, 46 (12). ISSN 0145-8884
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Official URL: http://doi.org/10.1111/jfbc.14487
Related URL: http://dx.doi.org/10.1111/jfbc.14487
Abstract
The epigallocatechin-rich polyphenolic fraction of Assam variety white tea, traditionally used for the management of diverse inflammatory ailments and health drink, was investigated through eco-friendly green aqueous extraction, TLC, and HPLC characterization, phytochemical screening, in vitro DPPH assay, anti-proteinase, MTT assay on synovial fibroblast and colon cancer cells, apoptotic FACS analysis, cytokine ELISA, p-STAT3 western blotting, and in silico docking analysis. HPLC-TLC standardized white tea fraction (WT-F) rendered higher extractive-yield (21%, w/w), than green tea fraction(GT-F) (12%, w/w). WT-F containing flavonoids and non-hydrolysable polyphenols showed better antioxidant activity, rather than equivalent GT-F. WT-F demonstrated remarkable anti-rheumatoid-arthritis activity via killing of synovial fibroblast cells (66.1%), downregulation of TNF-ɑ (93.33%), IL-6 (87.97%), and p-STAT3 inhibition (77.75%). Furthermore, WT-F demonstrated better anti-proliferative activity against colon cancer cells (HCT-116). Collectively, our study revealed that the white tea fraction has boundless potential as anti-rheumatoid arthritis and anti-proliferative agent coupled with apoptotic, antioxidant anti-proteinase, and anti-inflammatory properties.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons. |
ID Code: | 136128 |
Deposited On: | 20 May 2025 11:12 |
Last Modified: | 20 May 2025 11:12 |
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