Effect of internal irradiation on the maturing Purkinje cells in the rat: a Golgi study

Abstract

Continuous irradiation in utero is reported to produce mental retardation and gross abnormalities of the brain in the human. A few experimental studies conducted so far also report gross brain defects in animals exposed to continuous irradiation in utero. Despite the increasing use of nuclear energy for power and radioisotopes in medicine, there is hardly any literature available on the effect of continuous irradiation on the structural details of the developing brain. After intraperitoneal injections of different doses of ¹³¹I (8, 18 and 32 μCi) and ³²P (10 μCi) in new-born rats on the 6th postnatal day, cerebella stained by Golgi techniques were cut sagittally and the sections were examined on the 10th, 15th and 21st postnatal days. In the animals injected with 18 and 32 μCi of ¹³¹I and 10 μCi of ³²P a large number of Purkinje cells showed morphological alterations not seen in the control groups or in the groups injected with 8 μCi of ¹³¹I. The changes observed included persistence of the perisomatic processes beyond the 10th postnatal day, multiple primary dendrites, angulation of the primary dendrites, long segments of primary dendrites without branches and significantly reduced dendritic volume. The number of affected cells was less on the 21st postnatal day. The effective radiation dose estimated in these groups ranged from 15 to 26 rad. Since the rats irradiated with 6 rad had not shown such changes it is believed that there is a threshold dose of radiation beyond which only changes are perceptible at neuronal level.