Sarkar, Parijat ; Chattopadhyay, Amitabha (2020) Exploring Membrane Lipid and Protein Diffusion by FRAP Analysis of Membrane Lipids . pp. 119-141. ISSN 1949-2448
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Official URL: http://doi.org/10.1007/978-1-0716-0631-5_8
Related URL: http://dx.doi.org/10.1007/978-1-0716-0631-5_8
Abstract
Knowledge of membrane dynamics is crucial since it allows us to understand membrane function. Fluorescence recovery after photobleaching (FRAP) is a widely used technique to monitor diffusion of lipids and proteins in biological membranes. We outline here general aspects of FRAP, followed by a step-by-step guide to carry out FRAP measurements for exploring diffusion of fluorescently labeled lipids and proteins in membranes of attached cells and membranes of Candida albicans. In this process, we have provided detailed hands-on tips, judicious use of which would ensure reliability and quality of acquired FRAP data and associated analysis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Nature Switzerland AG |
| Keywords: | FRAP;Confocal microscopy;Lateral diffusion;Mobile fraction;Diffusion coefficient;DiI;GFP |
| ID Code: | 134950 |
| Deposited On: | 17 Jan 2023 05:10 |
| Last Modified: | 17 Jan 2023 05:10 |
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