Chattopadhyay, Amitabha ; Jafurulla, Md. (2014) Novel Insights in Membrane Biology Utilizing Fluorescence Recovery After Photobleaching Biochemical Roles of Eukaryotic Cell Surface Macromolecules, 842 . pp. 27-40. ISSN 0065-2598
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Official URL: http://doi.org/10.1007/978-3-319-11280-0_3
Related URL: http://dx.doi.org/10.1007/978-3-319-11280-0_3
Abstract
Understanding cellular signaling by membrane receptors in terms of their lateral dynamics represents a challenging area in contemporary biology. Fluorescence recovery after photobleaching (FRAP) offers a convenient approach to measure lateral diffusion and is extensively used for measuring lateral diffusion of lipids and proteins in membranes. In this review, we have provided an overview of the type of questions that could be addressed in membrane and receptor biology utilizing FRAP, with representative examples chosen from work carried out in our group. A major focus is on exploring new horizons in the organization and dynamics of G protein-coupled receptors (GPCRs) utilizing FRAP. We discuss how lateral dynamics of membrane receptors could serve as crucial determinants of their signaling. We envision that FRAP, along with confocal microscopy, could provide novel insight into dynamics of intracellular organelles.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer Nature Switzerland AG |
| Keywords: | Fluorescence Recovery After Photobleaching (FRAP);G Protein-coupled Receptors (GPCRs);FRAP Measurements;Bleached Area;Pucadyil |
| ID Code: | 134929 |
| Deposited On: | 17 Jan 2023 03:58 |
| Last Modified: | 17 Jan 2023 03:58 |
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