Moitra, Parikshit ; Misra, Santosh K. ; Kumar, Krishan ; Kondaiah, Paturu ; Tran, Phuong ; Duan, Wei ; Bhattacharya, Santanu (2021) Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes ACS Biomaterials Science & Engineering, 7 (6). pp. 2508-2519. ISSN 2373-9878
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Official URL: http://doi.org/10.1021/acsbiomaterials.1c00110
Related URL: http://dx.doi.org/10.1021/acsbiomaterials.1c00110
Abstract
A new pH-responsive cationic co-liposomal formulation was prepared in this study using the twin version of the amphiphile palmitoyl homocysteine, TPHC; natural zwitterionic lipid, DOPE; and cholesterol-based twin cationic lipid, C5C, at specified molar ratios. This co-liposome was further decorated with a newly designed fluorescently tagged, cholesterol-tethered EpCAM-targeting RNA aptamer for targeted gene delivery. This aptamer-guided nanoliposomal formulation, C5C/DOPE/TPHC at 8:24:1 molar ratio, could efficiently transport the genes in response to low pH of cellular endosomes selectively to the EpCAM overexpressing cancer stem cells. This particular observation was extended using siRNA against GFP to validate their transfection capabilities in response to EpCAM expression. Overall, the aptamer-guided nanoliposomal formulation was found to be an excellent transfectant for in vitro siRNA gene delivery.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Chemical Society. |
| ID Code: | 134283 |
| Deposited On: | 06 Jan 2023 03:53 |
| Last Modified: | 06 Jan 2023 03:53 |
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