Dhiman, Alisha ; Gopalani, Monisha ; Bhatnagar, Rakesh (2015) WalRK two component system of Bacillus anthracis responds to temperature and antibiotic stress Biochemical and Biophysical Research Communications, 459 (4). pp. 623-628. ISSN 0006-291X
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Official URL: http://doi.org/10.1016/j.bbrc.2015.02.159
Related URL: http://dx.doi.org/10.1016/j.bbrc.2015.02.159
Abstract
WalRK Two Component System (TCS) of Bacillus anthracis forms a functional TCS. This report elaborates upon the WalRK genomic architecture, promoter structure, promoter activity and expression under various stress conditions in B. anthracis. 5′ RACE located the WalRK functional promoter within 317 bp region upstream of WalR. Reporter gene assays demonstrated maximal promoter activity during early growth phases indicating utility in exponential stages of growth. qRT-PCR showed upregulation of WalRK transcripts during temperature and antibiotic stress. However, WalR overexpression did not affect the tested antibiotic MIC values in B. anthracis. Collectively, these results confirm that WalRK responds to cell envelope stress in B. anthracis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 133775 |
| Deposited On: | 30 Dec 2022 07:05 |
| Last Modified: | 30 Dec 2022 07:05 |
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