Sudhakara, Sneha ; Chadha, Anju (2015) A fourier transform infrared spectroscopy (FTIR) based assay for Candida parapsilosis ATCC 7330 mediated oxidation of aryl alcohols Journal of Biotechnology, 209 . pp. 102-107. ISSN 0168-1656
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Official URL: http://doi.org/10.1016/j.jbiotec.2015.06.398
Related URL: http://dx.doi.org/10.1016/j.jbiotec.2015.06.398
Abstract
We present an FTIR based assay to monitor the whole cell mediated oxidation of aryl alcohols by measuring the characteristic IR absorption of the hydroxyl group [OH] of the substrate and the carbonyl group [CO] of the corresponding oxidized product. This method expedites the analysis of whole cell mediated catalysis which is usually done by GC and/or HPLC. The FTIR assay had linearity with R(2)≥0.980 and sensitivity up to 10μM. The accuracy and precision of FTIR assay was found ≥81% and ≥94%, respectively. This assay was validated by GC which exhibited ≥82% accuracy and ≥79% precision. The time of analysis taken by this assay was 2-3min per sample in comparison with 20-40min by GC.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Biocatalytic oxidation; Candida parapsilosis ATCC 7330; FTIR spectroscopy; Gas chromatography. |
| ID Code: | 131929 |
| Deposited On: | 09 Dec 2022 10:14 |
| Last Modified: | 09 Dec 2022 10:14 |
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