Kumar, Vijay ; Bansal, Vidhu J. ; Rao, Kanury V. S. ; Jameel, Shahid (1992) Hepatitis B virus envelope epitopes: gene assembly and expression in Escherichia coli of an immunologically reactive novel multiple-epitope polypeptide 1 (MEP-1) Gene, 110 (2). pp. 137-144. ISSN 0378-1119
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/037811...
Related URL: http://dx.doi.org/10.1016/0378-1119(92)90640-B
Abstract
A novel synthetic 323-bp gene with the open reading frame of a multiple-epitope polypeptide has been assembled and cloned. The gene is engineered by contiguous alignment of selected epitopes and functional domains of the hepatitis B virus envelope proteins separated by pairs of glycine residues. High-level bacterial production of this 100-amino acid (approx. 10 kDa) protein has been achieved and the gene product is stable. ELISA and Western blot experiments using epitope-specific antisera confirm that the corresponding epitopes are present in the engineered protein.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Gene Synthesis; Recombinant DNA; Protein Engineering; ELISA; Western Immunoblot; Oligodeoxyribonucleotide; Surface Antigen |
| ID Code: | 13128 |
| Deposited On: | 11 Nov 2010 06:49 |
| Last Modified: | 03 Jun 2011 09:01 |
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