Sehgal, Deepak ; Malik, Punjab Singh ; Jameel, Shahid (2003) Purification and diagnostic utility of a recombinant hepatitis E virus capsid protein expressed in insect larvae Protein Expression and Purification, 27 (1). pp. 27-34. ISSN 1046-5928
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S10465...
Related URL: http://dx.doi.org/10.1016/S1046-5928(02)00574-0
Abstract
We report here the expression and purification of a truncated form of the hepatitis E virus ORF2 protein (ORF2Δ111/ΔTM), from the fat bodies of Spodoptera litura larvae infected with a recombinant baculovirus. The purified protein migrated as a doublet of ~56 kDa on SDS-PAGE and was found to be glycosylated by staining with concanavalin A-linked horseradish peroxidase. The protein was used in a sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to HEV. The results showed complete concordance with those obtained using a commercial kit for the detection of anti-HEV antibodies. Antigen expression in the insect larvae system presents a rapid and low-cost method that obviates the need for expensive tissue culture scale-ups or special equipment.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | AcMNPV; Baculovirus; Larvae; Hepatitis E Virus; ORF2 |
| ID Code: | 13081 |
| Deposited On: | 11 Nov 2010 06:54 |
| Last Modified: | 17 Feb 2011 06:37 |
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