Detection of the negative strand of hepatitis E virus RNA in the livers of experimentally infected rhesus Monkeys: evidence for viral replication

Nanda, Santosh Kumar ; Panda, Subrat Kumar ; Durgapal, Hemlata ; Jameel, Shahid (1994) Detection of the negative strand of hepatitis E virus RNA in the livers of experimentally infected rhesus Monkeys: evidence for viral replication Journal of Medical Virology, 42 (3). pp. 237-240. ISSN 0146-6615

Full text not available from this repository.

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jmv.189...

Related URL: http://dx.doi.org/10.1002/jmv.1890420306

Abstract

Hepatitis E virus (HEV), the causative agent of enteric non-A, non-B hepatitis, is a positive-stranded RNA virus. Because of the virus's inability to grow in culture, several nonhuman primates have been used for the propagation of HEV. Using strand-specific reverse transcription-polymerase chain reaction (RT-PCR), we demonstrate the presence of negative-stranded HEV RNA replicative intermediates in the livers of infected animals. This constitutes the first direct evidence of HEV replication in the liver of the infected animals and reinforces the validity of such a model to study HEV infection, disease pathogenesis, and immunity.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:HEV; RT-PCR; Animal Model
ID Code:13080
Deposited On:11 Nov 2010 06:54
Last Modified:23 Feb 2011 05:26

Repository Staff Only: item control page