Lal, Sunil K. ; Tulasiram, P. ; Jameel, Shahid (1997) Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris Gene, 190 (1). pp. 63-67. ISSN 0378-1119
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S03781...
Related URL: http://dx.doi.org/10.1016/S0378-1119(96)00698-1
Abstract
We have used the methylotrophic yeast, Pichia pastoris, to express the open reading frame 3 (ORF3) of the hepatitis E virus (HEV). The ORF3 gene codes for a 123-amino-acid protein that contains highly immunodominant epitopes and is a potentially useful diagnostic and immunoprophylactic antigen. The expressed protein showed positive on immunoblots probed against antibodies raised in rabbit and infected human patient sera. In order to optimize the ORF3 protein expression, we have examined the regulated expression of this protein and characterized it. Unlike its expression in E. coli, the ORF3 protein was present in both the soluble and insoluble fractions of the cell lysate. The expressed protein is not glycosylated and does not undergo any major processing in the host strain.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Alcohol Oxidase Promoter; Expression Plasmid; Integrative Transformation; Open Reading Frame; Heterologous Gene Expression; Methanol Utilization Pathway; Glycosylation Pattern |
| ID Code: | 13079 |
| Deposited On: | 11 Nov 2010 06:55 |
| Last Modified: | 17 Feb 2011 06:50 |
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