Singha, Monisha ; Kumar, Gaurav ; Jain, Diamond ; Kumar N, Ganesh ; Ray, Debashis ; Ghosh, Anindya S. ; Basak, Amit (2019) Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs ACS Omega, 4 (6). pp. 10891-10898. ISSN 2470-1343
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Official URL: http://doi.org/10.1021/acsomega.9b01145
Related URL: http://dx.doi.org/10.1021/acsomega.9b01145
Abstract
A method for rapid detection of metallo-β-lactamases NDM-5 and NDM-7 using conjugates of azidonaphthalimide and Zn(II) chelating motifs (like sulfonamides, hydroxamate, and terpyridine) is described. Incubation and irradiation, followed by gel electrophoresis, clearly show the presence of NDMs. The o-sulfonamide-based probe has the highest efficiency of detection for both the NDMs. The proteins are detectable at nM concentrations, and the method is also selective, works both in vitro and in vivo, as revealed by cellular imaging and also with clinical isolates.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Chemical Society. |
| ID Code: | 130067 |
| Deposited On: | 05 Dec 2022 05:49 |
| Last Modified: | 05 Dec 2022 05:49 |
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