Decrypting the oscillating nature of the 4′‐phosphopantetheine arm in acyl carrier protein AcpM of Mycobacterium tuberculosis

Biswas, Rupam ; Singh, Bina Kumari ; Dutta, Debajyoti ; Das, Prabir Kumar ; Maiti, Mrinal Kumar ; Basak, Amit ; Das, Amit Kumar (2019) Decrypting the oscillating nature of the 4′‐phosphopantetheine arm in acyl carrier protein AcpM of Mycobacterium tuberculosis FEBS Letters, 593 (6). pp. 622-633. ISSN 0014-5793

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Official URL: http://doi.org/10.1002/1873-3468.13339

Related URL: http://dx.doi.org/10.1002/1873-3468.13339

Abstract

In Mycobacterium tuberculosis, acyl carrier protein (AcpM)-mediated fatty acid synthase type II is integral for the synthesis of mycolic acids. AcpM, designated as an atypical ACP, comprises of a putative 33 amino acid long C-terminal extension which is distinctive in nature. Here, we aimed at devising an ‘easy-to-go’ method for the generation of crypto-AcpM loaded with a solvatochromic probe 7-Nitrobenz-2-oxa-1,3-diazol-4-yl, which is linked to the 4′-phosphopantetheine (Ppant) prosthetic group of AcpM. The crypto-AcpM, coupled with fluorescence spectroscopy and molecular dynamics simulation studies, was employed to explore the elusive dynamics of Ppant arm in AcpM. This investigation establishes the role of the flexible C-terminal extension of AcpM in regulating the prosthetic group sequestration ability by modulating the ‘Asp-Ser-Leu’ motif.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
ID Code:130066
Deposited On:05 Dec 2022 05:49
Last Modified:05 Dec 2022 05:49

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