Singh, Ravinder ; Kumar, Neeraj ; Bandopadhyay, Rajib ; Rustgi, Sachin ; Sharma, Shailendra ; Balyan, Harindra Singh ; Gupta, Pushpendra Kumar (2006) Development and use of anchored-SSRs to study DNA polymorphism in bread wheat (Triticum aestivum L.) Molecular Ecology Resources, 6 (1). pp. 296-299. ISSN 1755-098X
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...
Related URL: http://dx.doi.org/10.1111/j.1471-8286.2005.01202.x
Abstract
In bread wheat, 21 anchored simple sequence repeat (SSR) primer pairs detecting SSR length polymorphism and 42 anchored SSR primers detecting microsatellite-anchored fragment length polymorphisms (MFLPs) are reported. Eight bread wheat genotypes were used for detecting polymorphism. The number of alleles in SSR analysis ranged from two to six, with a mean of 2.9 alleles per SSR. The number of polymorphic bands in MFLP ranged from two to 40, with a mean of 12.74 polymorphic bands/primer combination, the SSRs with CT/GA motifs giving the highest level of polymorphism (a mean of 18.37 bands). The average value of polymorphic information content (PIC) was 0.473 for SSRs and 0.061 for MFLP.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
| Keywords: | Anchored-SSR; Bread Wheat; MFLP |
| ID Code: | 12873 |
| Deposited On: | 11 Nov 2010 08:40 |
| Last Modified: | 16 Feb 2011 03:46 |
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