Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer Cells

Nur, Suza Mohammad ; Shait Mohammed, Mohammed Razeeth ; Zamzami, Mazin A. ; Choudhry, Hani ; Ahmad, Aamir ; Ateeq, Bushra ; Rather, Irfan A. ; Khan, Mohammad Imran (2022) Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer Cells Metabolites, 12 (3). p. 267. ISSN 2218-1989

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Official URL: http://doi.org/10.3390/metabo12030267

Related URL: http://dx.doi.org/10.3390/metabo12030267

Abstract

Tumor cells detached from the extracellular matrix (ECM) undergo anoikis resistance and metabolic reprogramming to facilitate cancer cell survival and promote metastasis. During ECM detachment, cancer cells utilize genomic methylation to regulate transcriptional events. One-carbon (1C) metabolism is a well-known contributor of SAM, a global substrate for methylation reactions, especially DNA methylation. DNA methylation-mediated repression of NK cell ligands MICA and MICB during ECM detachment has been overlooked. In the current work, we quantitated the impact of ECM detachment on one-carbon metabolites, expression of 1C regulatory pathway genes, and total methylation levels. Our results showed that ECM detachment promotes the accumulation of one-carbon metabolites and induces regulatory pathway genes and total DNA methylation. Furthermore, we measured the expression of well-known targets of DNA methylation in NK cell ligands in cancer cells, namely, MICA/B, during ECM detachment and observed low expression compared to ECM-attached cancer cells. Finally, we treated the ECM-detached cancer cells with vitamin C (a global methylation inhibitor) and observed a reduction in the promoter methylation of NK cell ligands, resulting in MICA/B re-expression. Treatment with vitamin C was also found to reduce global DNA methylation levels in ECM-detached cancer cells.

Item Type:Article
Source:Copyright of this article belongs to MDPI
Keywords:one-carbon metabolism; ECM detachment; anoikis; DNA methylation; NKG2DLs
ID Code:127218
Deposited On:13 Oct 2022 09:16
Last Modified:13 Oct 2022 09:16

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