Roy, Jetendra K. ; Rai, Sudhir K. ; Mukherjee, Ashis K. (2012) Characterization and application of a detergent-stable alkaline α-amylase from Bacillus subtilis strain AS-S01a International Journal of Biological Macromolecules, 50 (1). pp. 219-229. ISSN 01418130
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Official URL: http://doi.org/10.1016/j.ijbiomac.2011.10.026
Related URL: http://dx.doi.org/10.1016/j.ijbiomac.2011.10.026
Abstract
A strain AS-S01a, capable of producing high-titer alkaline α-amylase, was isolated from a soil sample of Assam, India and was taxonomically identified as Bacillus subtilis strain AS-S01a. Optimized α-amylase yield by response surface method (RSM) was obtained as 799.0 U with a specific activity of 201.0 U/mg in a process control bioreactor. A 21.0 kDa alkaline α-amylase purified from this strain showed optimum activity at 55°C and pH 9.0, and it produced high molecular weight oligosaccharides including small amount of glucose from starch as the end product. The K(m) and V(max) values for this enzyme towards starch were determined as 1.9 mg/ml and 198.21 μmol/min/mg, respectively. The purified α-amylase retained its activity in presence of oxidant, surfactants, EDTA and various commercial laundry detergents, thus advocating its suitability for various industrial applications.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier B.V. |
| Keywords: | Alkaline α-amylase, Bacillus, Detergent compatibility, Response surface method, Submerged fermentation |
| ID Code: | 126845 |
| Deposited On: | 13 Oct 2022 07:04 |
| Last Modified: | 13 Oct 2022 07:04 |
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