Shukla, Arun Kumar ; Reinhart, Christoph ; Michel, Hartmut (2006) Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells FEBS Letters, 580 (17). pp. 4261-4265. ISSN 00145793
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Official URL: http://doi.org/10.1016/j.febslet.2006.05.064
Related URL: http://dx.doi.org/10.1016/j.febslet.2006.05.064
Abstract
High-level overexpression of G protein-coupled receptors GPCRs in mammalian cells remains a difficult task inspite of newly developed virus based expression systems. Here, we show that the functional expression level of the recombinant bradykinin receptor (B2R) in mammalian cells can be increased up to sixfold just by the addition of dimethylsulphoxide in the culture medium. Total expression level, cellular localization and binding affinity of the recombinant receptor for its endogenous ligand remains unaltered. The strategy presented here, with recombinant B2R as a case example, is applicable to other GPCRs and provides a generic tool to improve the functional expression level of recombinant GPCRs in mammalian cells.
Item Type: | Article |
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Source: | Copyright of this article belongs to Federation of European Biochemical Societies |
ID Code: | 126541 |
Deposited On: | 13 Oct 2022 06:46 |
Last Modified: | 13 Oct 2022 06:46 |
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