Malankar, Gauri S. ; Sakunthala, Arunima ; Navalkar, Ambuja ; Maji, Samir K. ; Raju, Saravanan ; Manjare, Sudesh T. (2021) Organoselenium-based BOPHY as a sensor for detection of hypochlorous acid in mammalian cells Analytica Chimica Acta, 1150 . p. 338205. ISSN 00032670
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Official URL: http://doi.org/10.1016/j.aca.2021.338205
Related URL: http://dx.doi.org/10.1016/j.aca.2021.338205
Abstract
Phenylselenide substituted BOPHY probes (BOPHY-SePh and PhSe-BOPHY-SePh) were synthesized and characterized by NMR spectroscopy and single-crystal XRD. Both the probes selectively detect HOCl in water with high sensitivity over other reactive oxygen species. A fluorescence “turn-on” event was attained due to cease of the PET process through transformation of selenide to selenoxide. Both the probes react with HOCl in less than 1 s. PhSe-BOPHY-SePh probe depicted low background fluorescence due to presence of two phenylselenide groups at BOPHY. PhSe-BOPHY-SePh probe has a low detection limit (0.63 μM) than BOPHY-SePh probe (1.08 μM). The bioimaging studies of both the probes were carried out in MCF 7 cells. Both the probes exhibited a good fluorescence response for HOCl in vitro and in mammalian cells. In addition, the probes showed reversibility with all bio-thiols, which was validated in MCF 7 cells using GSH.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier B.V |
| Keywords: | BOPHYF; luorescentprobes; Hypochlorousacid; Organoselenium;,Bioimaging; ROS |
| ID Code: | 126364 |
| Deposited On: | 31 Oct 2022 03:44 |
| Last Modified: | 31 Oct 2022 03:44 |
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