Methanocaldococcus jannaschii adenylosuccinate synthetase: Studies on temperature dependence of catalytic activity and structural stability

Abstract

Two important attributes of enzymes produced by thermophilic organisms are thermophilicity and structural stability. This manuscript discusses the characterization of these two aspects in adenylosuccinate synthetase from the thermophilic archaeon, Methanocaldococcus jannaschii. Adenylosuccinate synthetase catalyzes the formation of succinyl-AMP from IMP and aspartate with the simultaneous conversion of GTP to GDP. Temperature dependence of M. jannaschii AdSS (MjAdSS) catalysis exhibited a biphasic Arrhenius Plot with a transition at 40 °C. Pre-steady-state kinetics as a function of temperature indicated a change in rate determining step of the reaction across the inflection point. Slow release of products from the enzyme active site probably accounts for the thermophilicity of MjAdSS. Thermal unfolding of MjAdSS exhibited a T_m of 85degreeC, with the process being only partially reversible. Stability of MjAdSS assessed by equilibrium unfolding revealed the robustness of the secondary and tertiary structure of the enzyme which remained intact even at 8 M concentration of urea. Guanidinium chloride induced denaturation of MjAdSS permitted estimation of thermodynamic parameters. The unfolding profiles could be described as a composite of atleast two distinct transitions, with a stable intermediate in the unfolding pathway.