Comparative Studies of White-Rot Fungal Strains (Trametes hirsuta MTCC-1171 and Phanerochaete chrysosporium NCIM-1106) for Effective Degradation and Bioconversion of Ferulic Acid

Abstract

Biodegradation of ferulic acid, by two white-rot fungal strains (Trametes hirsuta MTCC-1171 and Phanerochaete chrysosporium NCIM-1106) was investigated in this study. Both strains could use ferulic acid as a sole carbon source when provided with basal mineral salt medium. T. hirsuta achieved complete degradation of ferulic acid (350 mg L–1) in 20 h, whereas P. chrysosporium degraded it (250 mg L–1) in 28 h. The metabolites produced during degradation were distinguished by gas chromatography–mass spectrometry. Bioconversion of ferulic acid to vanillin by P. chrysosporium was also investigated. The optimum experimental conditions for bioconversion to vanillin can be summarized as follows: ferulic acid concentration 250 mg L–1, temperature 35 °C, initial pH 5.0, mycelial inoculum 0.32 ± 0.01 g L–1 dry weight, and shaking speed 150 rpm. At optimized conditions, the maximum molar yield obtained was 3.4 ± 0.1%, after 20 h of bioconversion. Considering that the degradation of ferulic acid was determined by laccase and lignin peroxidase to some extent, the possible role of ligninolytic enzymes in overall bioconversion process was also studied. These results illustrate that both strains have the potential of utilizing ferulic acid as a sole carbon source. Moreover, P. chrysosporium can also be explored for its ability to transform ferulic acid into value-added products.