Azam, Sarwar ; Thakur, Vivek ; Ruperao, Pradeep ; Shah, Trushar ; Balaji, Jayashree ; Amindala, BhanuPrakash ; Farmer, Andrew D. ; Studholme, David J. ; May, Gregory D. ; Edwards, David ; Jones, Jonathan D. G. ; Varshney, Rajeev K. (2012) Coverage-based consensus calling (CbCC) of short sequence reads and comparison of CbCC results to identify SNPs in chickpea (Cicer arietinum; Fabaceae), a crop species without a reference genome American Journal of Botany, 99 (2). pp. 186-192. ISSN 0002-9122
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Official URL: http://doi.org/10.3732/AJB.1100419
Related URL: http://dx.doi.org/10.3732/AJB.1100419
Abstract
• Premise of the study: Next-generation sequencing (NGS) technologies are frequently used for resequencing and mining of single nucleotide polymorphisms (SNPs) by comparison to a reference genome. In crop species such as chickpea (Cicer arietinum) that lack a reference genome sequence, NGS-based SNP discovery is a challenge. Therefore, unlike probability-based statistical approaches for consensus calling and by comparison with a reference sequence, a coverage-based consensus calling (CbCC) approach was applied and two genotypes were compared for SNP identification.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley & Sons, Inc. |
| ID Code: | 125049 |
| Deposited On: | 27 Dec 2021 10:46 |
| Last Modified: | 27 Dec 2021 10:46 |
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