Maiti, S. ; Shear, Jason B. ; Williams, R. M. ; Zipfel, W. R. ; Webb, Watt W. (1997) Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation Science, 275 (5299). pp. 530-532. ISSN 0036-8075
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Official URL: http://doi.org/10.1126/science.275.5299.530
Related URL: http://dx.doi.org/10.1126/science.275.5299.530
Abstract
Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration (∼50 mM) and content (up to ∼5 × 108 molecules) of individual secretory granules.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Association for the Advancement of Science. |
| ID Code: | 123985 |
| Deposited On: | 26 Oct 2021 08:30 |
| Last Modified: | 26 Oct 2021 08:30 |
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