pH‐Dependent Nitrotyrosine Formation in Ribonuclease A is Enhanced in the Presence of Polyethylene Glycol (PEG)

Roy, Pritam ; Panda, Atashi ; Hati, Sumon ; Dasgupta, Swagata (2019) pH‐Dependent Nitrotyrosine Formation in Ribonuclease A is Enhanced in the Presence of Polyethylene Glycol (PEG) Chemistry - An Asian Journal, 14 (24). pp. 4780-4792. ISSN 1861-4728

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Official URL: http://doi.org/10.1002/asia.201901225

Related URL: http://dx.doi.org/10.1002/asia.201901225

Abstract

Protein nitration can occur as a result of peroxynitrite-mediated oxidative stress. Excess production of peroxynitrite (PN) within the cellular medium can cause oxidative damage to biomolecules. The in vitro nitration of Ribonuclease A (RNase A) results in nitrotyrosine (NT) formation with a strong dependence on the pH of the medium. In order to mimic the cellular environment in this study, PN-mediated RNase A nitration has been carried out in a crowded medium. The degree of nitration is higher at pH 7.4 (physiological pH) compared to pH 6.0 (tumor cell pH). The extent of nitration increases significantly when PN is added to RNase A in the presence of crowding agents PEG 400 and PEG 6000. PEG has been found to stabilize PN over a prolonged period, thereby increasing the degree of nitration. NT formation in RNase A also results in a significant loss in enzymatic activity.

Item Type:Article
Source:Copyright of this article belongs to John Wiley & Sons, Inc.
ID Code:123646
Deposited On:10 Oct 2021 09:14
Last Modified:10 Oct 2021 09:14

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