Sarkar, Deblina ; Chowdhury, Monalisa ; Das, Prasanta Kumar (2021) Naphthalimide based fluorescent organic nanoparticles in selective sensing of Fe3+ and as a diagnostic probe for Fe2+/Fe3+ transition Journal of Materials Chemistry B, 9 (2). pp. 494-507. ISSN 2050-750X
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Official URL: http://doi.org/10.1039/D0TB02450C
Related URL: http://dx.doi.org/10.1039/D0TB02450C
Abstract
Fluorescent organic nanoparticles (FONPs) have attracted considerable attention as a practical and effective platform for sensing and imaging applications. The present article delineates the fabrication of FONPs derived from the naphthalimide based histidine appended amphiphile, NID. The self-assembly of NID in 99 vol% water in DMSO led to the formation of FONPs through J-type aggregation. Aggregation-induced emission (AIE) was observed due to the pre-associated excimer of NID with bluish green emission at 470 nm along with intramolecular charge transfer (ICT). The emission of NID FONPs was utilized for selective sensing of Fe3+ and bioimaging of Fe3+ inside mammalian cells. The fluorescence intensity of the FONPs was quenched with the gradual addition of Fe3+ due to the formation of a 1 : 1 stoichiometric complex with the histidine residue of NID. The morphology of the FONPs transformed from spherical to spindle upon the complex formation of NID with Fe3+. The limit of detection (LOD) of this AIE based turn-off chemosensor for Fe3+ was found to be 12.5 ± 1.2 μM having high selectivity over other metal ions. On the basis of the very low cytotoxicity and selective sensing of Fe3+, NID FONPs were successfully employed for bioimaging of Fe3+ ions through fluorescence quenching within mammalian cells (NIH3T3, B16F10). Considering the varying oxidative stress inside different cells, NID FONPs were used for detecting Fe2+ to Fe3+ redox state transition selectively inside cancer cells (B16F10) in comparison to non-cancerous cells (NIH3T3). Selective sensing of cancer cells was substantiated by co-culture experiment and flow cytometry. Hence, NID FONPs can be a selective diagnostic probe for cancer cells owing to their higher H2O2 content.
Item Type: | Article |
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Source: | Copyright of this article belongs to Royal Society of Chemistry. |
ID Code: | 123621 |
Deposited On: | 08 Oct 2021 06:34 |
Last Modified: | 08 Oct 2021 06:34 |
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