Patchornik, Guy ; Namboothiri, Irishi N.N. ; Nair, Divya K. ; Wachtel, Ellen ; Cohen, Sidney R. ; Friedman, Noga ; Sheves, Mordechai (2012) Engineered-membranes: A novel concept for clustering of native lipid bilayers Journal of Colloid and Interface Science, 388 (1). pp. 300-305. ISSN 0021-9797
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Official URL: http://doi.org/10.1016/j.jcis.2012.08.024
Related URL: http://dx.doi.org/10.1016/j.jcis.2012.08.024
Abstract
A strategy for clustering of native lipid membranes is presented. It relies on the formation of complexes between hydrophobic chelators embedded within the lipid bilayer and metal cations in the aqueous phase, capable of binding two (or more) chelators simultaneously Fig. 1. We used this approach with purple membranes containing the light driven proton pump protein bacteriorhodopsin (bR) and showed that patches of purple membranes cluster into mm sized aggregates and that these are stable for months when incubated at 19°C in the dark. The strategy may be general since four different hydrophobic chelators (1,10-phenanthroline, bathophenanthroline, Phen-C10, and 8-hydroxyquinoline) and various divalent cations (Ni2+, Zn2+, Cd2+, Mn2+, and Cu2+) induced formation of membrane clusters. Moreover, the absolute requirement for a hydrophobic chelator and the appropriate metal cations was demonstrated with light and atomic force microscopy (AFM); the presence of the metal does not appear to affect the functional state of the protein. The potential utility of the approach as an alternative to assembled lipid bilayers is suggested.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
ID Code: | 121567 |
Deposited On: | 19 Jul 2021 09:15 |
Last Modified: | 19 Jul 2021 09:15 |
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