Sreenivas, K. ; Ganguly, Nirmal K. ; Ghosh, Sujata ; Sehgal, Rakesh ; Mahajan, Ramesh C. (1995) Identification of a 148-kDa surface lectin from Giardia lamblia with specificity for α-methyl-d-mannoside FEMS Microbiology Letters, 134 (1). pp. 33-37. ISSN 0378-1097
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...
Related URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07910.x
Abstract
A lectin specific for α-methyl-d-mannoside was purified from the membrane extract of Giardia lamblia by a combination of gel filtration chromatography on Sephadex G-75 and Superose 6-HR 10/30. The homogeneity of the lectin was established by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular mass of the native protein was 148 kDa. The lectin agglutinated rabbit erythrocytes in the presence of Ca2+ at 37 °C and pH 7.O. The maximum activity of the lectin was obtained after trypsin treatment. The inhibition study clearly suggests that the binding site of the lectin recognizes α-methyl-d-mannoside as the immunodominant sugar.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Microbiological Societies. |
| Keywords: | Lectin; Giardia Lamblia; Hemagglutinin; Agglutinin; α-Methyl-d-mannoside; Hemagglutination |
| ID Code: | 12118 |
| Deposited On: | 10 Nov 2010 04:27 |
| Last Modified: | 31 May 2011 10:29 |
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