Manderwad, Guru Prasad ; Gokul, Gopinathan ; Kannabiran, Chitra ; Honavar, Santosh G. ; Khosla, Sanjeev ; Vemuganti, Geeta K. (2010) Hypomethylation of the DNMT3L Promoter in Ocular Surface Squamous Neoplasia Archives of Pathology & Laboratory Medicine, 134 (8). pp. 1193-1196. ISSN 0003-9985
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Official URL: http://doi.org/10.5858/2009-0417-OA.1
Related URL: http://dx.doi.org/10.5858/2009-0417-OA.1
Abstract
Context: Cancer is known to have epigenetic inputs, with events like genomewide hypomethylation and gene-specific hypermethylation of DNA. The DNA methyltransferase enzymes act as effectors of this reprogramming. A previous study revealed that hypomethylation at the DNA methyltransferase 3-like (DNMT3L) promoter could be a potential biomarker in cervical tumors. Because the pathobiology of ocular surface squamous neoplasia (OSSN) is similar to that of cervical tumors, we wanted to determine whether similar changes occur in the methylation pattern at the DNMT3L promoter in OSSN. Objective: To evaluate the methylation status of the DNMT3L promoter in OSSN compared with healthy conjunctiva. Design: We evaluated DNA methylation at the DNMT3L promoter in the tumor tissues of 6 patients with histologically proven OSSN and in healthy conjunctiva tissue from 7 individuals for controls using the sodium bisulfite-assisted conversion of genomic DNA. Extracted genomic DNA was treated with sodium bisulfite and amplified with specific primers for the DNMT3L promoter region. The specific polymerase chain reaction products were cloned and sequenced. Results: The mean age of these patients was 50.2 years (range, 35-65 years). Histologically, 4 OSSN cases were invasive; 2 were intraepithelial. Healthy conjunctival tissues exhibited a methylated promoter region, whereas a variable loss of methylation was observed in all 6 OSSN cases. Conclusions: We have, for the first time to our knowledge, identified loss of methylation at the DNMT3L promoter in OSSN cases, but its physiologic significance is yet to be understood. Further studies are warranted to substantiate our results.
Item Type: | Article |
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Source: | Copyright of this article belongs to College of American Pathologists. |
ID Code: | 119931 |
Deposited On: | 18 Jun 2021 13:01 |
Last Modified: | 18 Jun 2021 13:01 |
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