Tripathi, Anil K. ; Tripathi, Rachna ; Ganguli, Abhijit ; Bazzicalupo, Marco (1998) Duplication of insertion element IS50 associated with Tn5 transposition in Azospirillum brasilense Canadian Journal of Microbiology, 44 (11). pp. 1110-1113. ISSN 0008-4166
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Official URL: http://doi.org/10.1139/cjm-44-11-1110
Related URL: http://dx.doi.org/10.1139/cjm-44-11-1110
Abstract
The characterization of a DNA fragment with a Tn5 insertion in a regulatory nif gene of Azospirillum brasilense is reported. Restriction endonuclease mapping, Southern hybridization with a Tn5 probe, and nucleotide sequencing revealed that IS50 had duplicated in Tn5. The duplication of an IS50 element suggests the occurrence of a replicative mechanism of transposition. A strategy, based on the bacterial ability of homologous recombination that was used to precisely eliminate Tn5 along with the duplicated IS50 element, is presented.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Canada Institute for Scientific and Technical Information. |
| ID Code: | 119206 |
| Deposited On: | 09 Jun 2021 05:12 |
| Last Modified: | 09 Jun 2021 05:12 |
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