Chakravortty, Dipshikha ; Koide, Naoki ; Kato, Yutaka ; Sugiyama, Tsuyoshi ; Kawai, Makoto ; Fukada, Masako ; Yoshida, Tomoaki ; Yokochi, Takashi (2000) Cytoskeletal Alterations in Lipopolysaccharide-Induced Bovine Vascular Endothelial Cell Injury and Its Prevention by Sodium Arsenite Clinical and Vaccine Immunology, 7 (2). pp. 218-225. ISSN 1071-412X
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Official URL: http://doi.org/10.1128/CDLI.7.2.218-225.2000
Related URL: http://dx.doi.org/10.1128/CDLI.7.2.218-225.2000
Abstract
Morphological changes, especially cytoskeletal alterations, in lipopolysaccharide (LPS)-induced vascular endothelial cell injury were studied by using LPS-susceptible bovine aortic endothelial cells (BAEC). BAEC in cultures with LPS showed cell rounding, shrinking, and intercellular gap formation. In those cells, LPS caused the disorganization of actin, tubulin, and vimentin. LPS also induced a reduction in the F-actin pool and an elevation in the G-actin pool. Cytoskeletal disorganization affected transendothelial permeability across the endothelial monolayer. Pretreatment of BAEC with sodium arsenite (SA) prevented alterations in LPS-induced BAEC injury. However, posttreatment with SA had no protective effect on them. SA upregulated the expression of heat shock protein in the presence of LPS. The role of SA in prevention of LPS-induced BAEC injury is discussed.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society for Microbiology. |
ID Code: | 118464 |
Deposited On: | 21 May 2021 09:06 |
Last Modified: | 02 Feb 2023 09:45 |
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