Koide, Naoki ; Sugiyama, Tsuyoshi ; Kato, Yutaka ; Chakravortty, Dipshika ; Mya Mya Mu, . ; Yoshida, Tomoaki ; Hamano, Teruaki ; Yokochi, Takashi (2001) Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14 Journal of Endotoxin Research, 7 (1). pp. 39-43. ISSN 0968-0519
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Official URL: http://doi.org/10.1177/09680519010070010601
Related URL: http://dx.doi.org/10.1177/09680519010070010601
Abstract
The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-κB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.
Item Type: | Article |
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Source: | Copyright of this article belongs to SAGE Publications Ltd. |
ID Code: | 118456 |
Deposited On: | 21 May 2021 08:43 |
Last Modified: | 02 Feb 2023 09:36 |
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