14-3-3γ meditated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo

Sehgal, Lalit ; Mukhopadhyay, Amitabha ; Rajan, Anandi ; Khapare, Nileema ; Sawant, Mugdha ; Vishal, Sonali S. ; Bhatt, Khyati ; Ambatipudi, Srikant ; Antao, Noelle ; Alam, Hunain ; Gurjar, Mansa ; Basu, Srikanta ; Mathur, Rohit ; Borde, Lalit ; Hosing, Amol S. ; Vaidya, Milind M. ; Thorat, Rahul ; Samaniego, Felipe ; Kolthur-Seetharam, Ullas ; Dalal, Sorab N. (2014) 14-3-3γ meditated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo Journal of Cell Science, 127 (10). pp. 2174-2188. ISSN 0021-9533

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Official URL: http://doi.org/10.1242/jcs.125807

Related URL: http://dx.doi.org/10.1242/jcs.125807

Abstract

The regulation of cell-cell adhesion is important for the processes of tissue formation and morphogenesis. Here, we report that loss of 14-3-3γ leads to a decrease in cell-cell adhesion and a defect in the transport of plakoglobin and other desmosomal proteins to the cell border in HCT116 cells and cells of the mouse testis. 14-3-3γ binds to plakoglobin in a PKCμ-dependent fashion, resulting in microtubule-dependent transport of plakoglobin to cell borders. Transport of plakoglobin to the border is dependent on the KIF5B-KLC1 complex. Knockdown of KIF5B in HCT116 cells, or in the mouse testis, results in a phenotype similar to that observed upon 14-3-3γ knockdown. Our results suggest that loss of 14-3-3γ leads to decreased desmosome formation and a decrease in cell-cell adhesion in vitro, and in the mouse testis in vivo, leading to defects in testis organization and spermatogenesis.

Item Type:Article
Source:Copyright of this article belongs to The Company of Biologists.
Keywords:14-3-3γ; Desmosome; KIF5B; Plakoglobin; Spermatogenesis.
ID Code:118214
Deposited On:19 May 2021 07:42
Last Modified:19 May 2021 07:42

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