Bais, Sachendra S. ; Ratra, Yashika ; Khan, Naseem A. ; Pandey, Rakesh ; Kushawaha, Pramod K. ; Tomar, Shailly ; Medigeshi, Guruprasad ; Singh, Abhyudai ; Basak, Soumen ; Williams, Bryan R. G. (2019) Chandipura Virus Utilizes the Prosurvival Function of RelA NF-κB for Its Propagation Journal of Virology, 93 (14). ISSN 0022-538X
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Official URL: http://doi.org/10.1128/JVI.00081-19
Related URL: http://dx.doi.org/10.1128/JVI.00081-19
Abstract
Chandipura virus (CHPV), a cytoplasmic RNA virus, has been implicated in several outbreaks of acute encephalitis in India. Despite the relevance of CHPV to human health, how the virus interacts with the host signaling machinery remains obscure. In response to viral infections, mammalian cells activate RelA/NF-κB heterodimers, which induce genes encoding interferon beta (IFN-β) and other immune mediators. Therefore, RelA is generally considered to be an antiviral transcription factor. However, RelA activates a wide spectrum of genes in physiological settings, and there is a paucity of direct genetic evidence substantiating antiviral RelA functions. Using mouse embryonic fibroblasts, we genetically dissected the role of RelA in CHPV pathogenesis. We found that CHPV indeed activated RelA and that RelA deficiency abrogated the expression of IFN-β in response to virus infections. Unexpectedly, infection of Rela−/− fibroblasts led to a decreased CHPV yield. Our investigation clarified that RelA-dependent synthesis of prosurvival factors restrained infection-inflicted cell death and that exacerbated cell death processes prevented multiplication of CHPV in RelA-deficient cells. Chikungunya virus, a cytopathic RNA virus associated also with epidemics, required RelA, and Japanese encephalitis virus, which produced relatively minor cytopathic effects in fibroblasts, circumvented the need of RelA for their propagation. In sum, we documented a proviral function of the pleiotropic factor RelA linked to its prosurvival properties. RelA promoted the growth of cytopathic RNA viruses by extending the life span of infected cells, which serve as the replicative niche of intracellular pathogens. We argue that our finding bears significance for understanding host-virus interactions and may have implications for antiviral therapeutic regimes.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society for Microbiology. |
Keywords: | Cytopathic; Interferon; NF-κB; RNA Virus; RelA; Burst Size; Cell Death. |
ID Code: | 115254 |
Deposited On: | 17 Mar 2021 04:33 |
Last Modified: | 17 Mar 2021 04:33 |
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