Cloning and sequence analysis of b-tubulin gene of Trypanosoma evansi isolates from Indian dromedaries (Camelus dromedarius)

Abstract

Trypanosomosis caused by Trypanosoma evansi is a disease of economic significance of domestic livestock, causing trypanosomiasis, commonly known as Surra. In the present study, gene of Trypanosoma evansi have beer cloned and sequenced. Beta-tubulin is important for cellular structure and physical functions. Sequence analysis of this gene will be useful for the vaccine studies. The open reading frame of b-tubulin of Trypanosoma evansi is 1329 bp in length, encoding 442 amino acid polypeptide. The sequence analysis revealed that the ß tubulin gene of Trypanosoma evansi from India was having the identity of 99% with T.equiperdum, T.evansi (China isolate), T.evansi (Indian isolates), T.brucei and 88% with T.cruzi. The stable biological characteristics of Trypanosomatidae beta-tubulin make it an ideal target of many anti-parasitic drugs that block tubulin function.