Chakka, Deviprasanna ; Gudla, Ramurthy ; Madikonda, Ashok Kumar ; Pandeeti, Emmanuel Vijay Paul ; Parthasarathy, Sunil ; Nandavaram, Aparna ; Siddavattam, Dayananda (2015) The Organophosphate degradation (opd) island-borne esterase-induced metabolic diversion in Escherichia coli and its influence on p-nitrophenol degradation Journal of Biological Chemistry, 290 (50). pp. 29920-29930. ISSN 0021-9258
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Official URL: http://www.jbc.org/content/early/2015/10/09/jbc.M1...
Related URL: http://dx.doi.org/10.1074/jbc.M115.661249
Abstract
In previous studies of the organophosphate degradation gene cluster we showed that expression of an open reading frame (orf306) present within the cluster in E. coli allowed growth on p-nitrophenol (PNP) as sole carbon source. We have now shown that expression of orf306 in E. coli causes a dramatic up-regulation in genes coding for alternative carbon catabolism. The propionate, glyoxylate and Methyl Citrate Cycle (MCC) pathway-specific enzymes are up regulated, along with hca (phenyl propionate) and mhp (hydroxy phenyl propionate) degradation operons. These hca and mhp operons play a key role in degradation of PNP, enabling E. coli to grow using it as sole carbon source. Supporting growth experiments, PNP degradation products entered central metabolic pathways and got incorporated into the carbon backbone. The protein and RNA samples isolated from E. coli (pSDP10) cells grown in C14 labelled PNP indicated incorporation of C14 carbon suggesting Orf306-dependent assimilation of PNP in E. coli cells.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society for Biochemistry and Molecular Biology. |
Keywords: | Biodegradation; Escherichia Coli (E. Coli); Gene Expression; Gene Knockout; Metabolic Regulation; Microarray |
ID Code: | 114194 |
Deposited On: | 28 May 2018 08:35 |
Last Modified: | 28 May 2018 08:35 |
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