Expression and subcellular localization of organophosphate hydrolase in acephate-degrading Pseudomonas sp. strain Ind01 and its use as a potential biocatalyst for elimination of organophosphate insecticides

Pinjari, A. B. ; Pandey, J. P. ; Kamireddy, S. ; Siddavattam, D. (2013) Expression and subcellular localization of organophosphate hydrolase in acephate-degrading Pseudomonas sp. strain Ind01 and its use as a potential biocatalyst for elimination of organophosphate insecticides Letters in Applied Microbiology, 57 (1). pp. 63-68. ISSN 0266-8254

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Official URL: https://onlinelibrary.wiley.com/doi/full/10.1111/l...

Related URL: http://dx.doi.org/10.1111/lam.12080

Abstract

Organophosphate hydrolase (OPH), the product of an organophosphate‐degrading (opd) gene cloned from Brevundimonas diminuta, hydrolyses the triester linkage found in neurotoxic organophosphate (OP) insecticides and nerve agents. Despite the fact that OPHs have a broad substrate range, OP compounds with a P‐S linkage, such as insecticides like acephate, are poor substrates for the enzyme. Expression of OPH in acephate‐utilizing Pseudomonas sp. Ind01 generated a live biocatalyst capable of degrading a wide range of OP insecticides. The heterologously expressed OPH, which is a substrate of twin arginine transport (Tat) pathway, successfully targeted to the membrane of Pseudomonas sp. Ind01. The membrane‐associated OPH had a size that coincided with the mature form of OPH (mOPH), suggesting successful processing and targeting of the expressed OPH to the membrane. Pseudomonas sp. Ind01 expressing OPH degraded a variety of OP insecticides besides using acephate as sole carbon source.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Biocatalyst; Biodegradation; Nerve Agents; Organophosphate Degradation Gene; Organophosphate Hydrolase
ID Code:114180
Deposited On:28 May 2018 08:05
Last Modified:28 May 2018 08:05

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