Dasgupta, Swagata ; Iyer, Ganesh H. ; Bryant, Stephen H. ; Lawrence, Charles E. ; Bell, Jeffrey A. (1997) Extent and nature of contacts between protein molecules in crystal lattices and between subunits of protein oligomers Proteins: Structure, Function, and Bioinformatics, 28 (4). pp. 494-514. ISSN 0887-3585
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Official URL: https://onlinelibrary.wiley.com/doi/pdf/10.1002/%2...
Related URL: http://dx.doi.org/10.1002/(SICI)1097-0134(199708)28:4<494::AID-PROT4>3.0.CO;2-A
Abstract
A survey was compiled of several characteristics of the intersubunit contacts in 58 oligomeric proteins, and of the intermolecular contacts in the lattice for 223 protein crystal structures. The total number of atoms in contact and the secondary structure elements involved are similar in the two types of interfaces. Crystal contact patches are frequently smaller than patches involved in oligomer interfaces. Crystal contacts result from more numerous interactions by polar residues, compared with a tendency toward nonpolar amino acids at oligomer interfaces. Arginine is the only amino acid prominent in both types of interfaces. Potentials of mean force for residue–residue contacts at both crystal and oligomer interfaces were derived from comparison of the number of observed residue–residue interactions with the number expected by mass action. They show that hydrophobic interactions at oligomer interfaces favor aromatic amino acids and methionine over aliphatic amino acids; and that crystal contacts form in such a way as to avoid inclusion of hydrophobic interactions. They also suggest that complex salt bridges with certain amino acid compositions might be important in oligomer formation. For a protein that is recalcitrant to crystallization, substitution of lysine residues with arginine or glutamine is a recommended strategy.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
Keywords: | Potential of Mean Force; Molecular Recognition; Protein Interfaces; Salt Bridges; Hydrophobic Interaction; Protein Crystallization; Contact Patches |
ID Code: | 113997 |
Deposited On: | 15 May 2018 06:48 |
Last Modified: | 15 May 2018 06:48 |
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