Methyl thiophanate as a DNA minor groove binder produces MT–Cu(II)–DNA ternary complex preferably with AT rich region for initiation of DNA damage

Saquib, Quaiser ; Al-Khedhairy, Abdulaziz A. ; Alarifi, Saud A. ; Dutta, Sansa ; Dasgupta, Swagata ; Musarrat, Javed (2010) Methyl thiophanate as a DNA minor groove binder produces MT–Cu(II)–DNA ternary complex preferably with AT rich region for initiation of DNA damage International Journal of Biological Macromolecules, 47 (1). pp. 68-75. ISSN 0141-8130

Full text not available from this repository.

Official URL: https://www.sciencedirect.com/science/article/pii/...

Related URL: http://dx.doi.org/10.1016/j.ijbiomac.2010.03.017

Abstract

Interaction of a genotoxic fungicide methyl thiophanate (MT) has been studied in vitro with calf thymus DNA. Fluorescence quenching data revealed the binding constant (Ka = 3.23 × 104 M−1) and binding capacity (n = 1.1) of MT with ctDNA. Ligand displacement studies using specific probes suggested the MT binding at DNA minor groove. The docking analysis further substantiated MT interaction with at least three AT base pairs within the DNA groove. A discernable change in E0′ value with decreased peak currents in cyclic voltammogram, and peak shifts in CD spectra reflected the formation of MT–ctDNA and MT–ctDNA–Cu(II) complexes. The results elucidate the significance of specific MT–DNA interactions as an initiating event in MT-induced DNA damage.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Fungicide; Fluorescence Quenching; Methyl Thiophanate; Circular Dichroism; Cyclic Voltammetry; DNA Binding
ID Code:113771
Deposited On:15 May 2018 06:17
Last Modified:15 May 2018 06:17

Repository Staff Only: item control page